Gastrointestinal nematodes (GIN) cost the sheep industry $369 million annually. With anthelmintic resistance and no commercialised vaccine, economical control strategies will require a further understanding of immune resistance development in sheep. Galectin-11 is a protein secreted into the mucus during GIN infections. Our work has shown an inverse correlation between sheep galectin-11 production and Haemonchus contortus (H. contortus) infection suggesting its role as a marker of GIN resistant sheep. However, the role of galectin-11 in GIN resistance is unknown therefore in the present study we used the larval migration assay (LMA) and immunohistochemistry to determine the effect of galectin-11 on H. contortus larval stages. LMA results showed no clear effect of galectin-11 on exsheathed L3 migration. However, immunohistochemistry results showed specific binding of galectin-11 to the surface of L4. This work suggests that galectin-11 may have a potential role in modulating L4 H. contortus activity and future studies are under way. Understanding the function of galectin-11 will assist in understanding the mechanisms of GIN immune resistance in sheep.