No specific reports exist on the presence of members of the family Pasteurellaceae in macropods. This initial investigation examined free wild kangaroos for the presence of Pasteurellaceae like bacteria and then undertook initial characterization tests.  A total of 98 isolates of Pasteurellaceae were obtained from oropharyngeal swabs collected from legally harvested wild kangaroos in southern Queensland. The 98 isolates were characterized by partial rpoB sequence analysis and formed 3 groups (55 isolates in Group 1, 21 in Group 2 and 22 in Group 3). 16S rRNA sequencing of 18 selected strains showed a monophyletic but diverse group (>94.8% within group similarity) which appear to represent novel genera and species. Existing close relatives were as follows:- Group 1 - Actinobacillus lignieresii (95.6%) and Mannheimia caviae (95.5%); Group 2 - A. lignieresii (95.6%) and [A.] minor (96.6%); Group 3 - A. arthritidis (95.4%). Phenotypical characterization (32 tests) showed that 30 selected strains from the 3 groups were Gram negative, oxidase and nitrate positive and catalase negative. Satellitism was variable between within the groups. Separation of the 3 groups was not fully achieved. Some of the isolates demonstrated a high level V-factor requirement and special methods were required for the characterization work.